LEE Broth – Listeria Express Enrichment Broth

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Description

Listeria Express Enrichment Broth (LEE Broth)

is aselective enrichment broth for the detection ofListeria. Developed to give improved growth rates ofListeriaover traditional selective enrichment media,

LEE Broth enhances the expression of target antigens for most commercially available immunological test kits/methods whilst maintaining adequate suppression of potential non-target organisms.

Selective components are blended into the powder, removing the requirement for

supplementation.

Compared with more traditional methods, immunological tests, such as ELISA, require relatively high levels of target organisms to achieve a reliable positive result. LEE Broth has been specifically designed to stimulate growth from low numbers to the required high levels within a 24 hour period.

Therefore, LEE Broth offers

an excellent choice for laboratories employing

immunological testmethods for detection of

Listeriain food products.

Lab M LEE Broth can be used as an

enrichment broth prior to plating on selective media such asHarlequin™ Listeria ChromogenicAgar ISO (HAL010 / PIN001);

as secondary selective enrichment medium

following primary enrichment in,

for example,HalfFraser Broth(LAB211 or LAB164); oras the enrichment step of a rapid method e.g. ELISA,lateral flow device, PCR

Formulation g/litre

Peptone 13.0

Growth enhancers 8.0

Buffer 22.2

Selective mix 3.0

Grams per litre 46.2

Appearance Powder:  fine, free-flowing, homogeneous, buff

Finished medium: clear, dark straw liquid with yellow fluorescence

pH:7.2 ± 0.1

Hazard classification Xi –Irritant

Method for reconstitution

Disperse 46.2g of powder in 1 litre of distilled water.

Allow to soak for 10 minutes, swirl to mix and dispense into suitable containers.

Sterilise by autoclaving for 15 minutes at 121oC. Allow to cool to ambient temperature prior to use.

Incubation

Incubate at 30oC +1oC for 24+1 hour

(for optimum performance check at 24 hours precisely).

Alternative incubation temperatures may be used if flagella are not the target antigen.

Interpretation

Low cell numbers may express high levels of detection targets therefore further tests (plating, ELISA etc) should be conducted on all samples regardless of presence/absence of turbidity.